Department of Biological Sciences
College of Science and Mathematics
Iligan Institute of Technology
Iligan City
COURSE SYLLABUS
For the First Semester 2010-2011
Buenaflor D. Jimenez
Email : bdjimenez7@gmail.com
I. Course Number: Bio 170.1
II. Course Title Laboratory in Marine Microbiology
III. Course Description
IV. Course Credit 1 unit: 3 hours laboratory/week
V. Course Pre-requisite: Bio 102, Bio 103 and Bio 106
VI. General Objective
VII. Specific Objective
1) To familiarize students with microbiology laboratory techniques, procedure and equipment that is commonly employed in marine microbiology.
2) Expose students to selected methods of evaluating and presenting scientific results
3) Areas covered include microscopy, functional activities of various microorganisms.
4) To identify the different requirements for growth and to describe the several direct and indirect measurements of growth and categorize the different nutritional patterns among organisms.
5) To know the beneficial activities of various microorganisms
.
VIII. Course Content:
June 17, 2010
Lab Exercise No 1. Microscopy
June 24, 2010
Lab Exercise No. 2. Use of Microscope in Microbiology
Lab Exercise 3 Winogradsky Column
July 01, 2010
Lab Exercise No 3 Preparation of Media
Due date for Lab Exercise No 1
July 08, 2010 Lab Exercise No. 4 Functional Groups of Bacteria
July 15, 2010
Lab Exercise No. 5. Microbial Biofilm
Due date for Lab Exercise 2
July 22, 2010
Lab Exercise No. 6 Microscopic Appearance of Microorganism
Due date for Lab Exercise 3
July 29, 2010
Lab Exercise No 7 Direct Examination of Unstained Bacteria
Due date for Lab Exercise 4
Aug 05, 2010
Lab Exercise No. 8 Biochemical Reactions of Bacteria
Due date for Lab Exercise No 5
Aug 12, 2010 Lab Exercise 9. Effects of Physical Factors on Survival and Growth of Microorganisms
Due date for Lab Exercise 6
Aug 19, 2010 Lab Exercise 10. Bioremediation of Gasoline
Due date for Lab Exercise 7
Aug 26, 2010 Due date for Lab Exercise 8
Sept 09, 2010 Due date for Lab Exercise 9
Sept 16, 2010 Due date for Lab Exercise 10
IX. Course Assessment :
Grade Evaluation
There are ten laboratory exercises scheduled in the term. All laboratories are mandatory. Failure to complete 80% of the laboratories may not be given a passing grade. Laboratories cannot be made.
Any students who cannot attend a laboratory session must request permission to submit any corresponding written assignments.
Data Notebooks
All students are required to keep records of all laboratory data which will be submitted after the end of the lab period.
Lab performance
All students are graded individually on their ability to perform in the laboratory. Although students are not assessed by a perfect performance, they will be evaluated on their preparedness, ability to execute labs independently, time on task as well as cleanliness and organization.
Laboratory reports are required for all laboratory exercises following the general format of a research paper. Please refer to the handout “Writing Scientific Reports” and “How to analyze and present data” for tips on making graphs and tables to present your data. All scientific reports are due a week after the return of your data notebooks. Late reports are deducted 5% per day and be accepted after the original due date.
Student Evaluation
Scientific paper/ Reports 70%
Data Notebook 20%
Lab performance/Attendance 10%
Total 100%
X. Guidelines and Policies regarding activity/ assignment submission/ Attendance Policy
Regular attendance is required at all class meetings and laboratory exercises and field trips. Laboratory exercises and field trips cannot be repeated for student’s that miss them. Students must contact me in advance if they must miss an activity so that alternative assignments will be made.
Scholastic Dishonesty policy
Scholastic dishonesty will not be tolerated
XI. Course Materials
Anderson, Douglas. 1974. Fish Immunology. In Sneiszco and Axelrod (eds). Diseases of Fishes. Neptune New Jersey: TFH Publications, Inc Ltd. 237pp.
Austin, B. Marine Microbiology. 1988. Cambridge: Cambridge University Press. 222pp.
Austin, B (ed) 1988. Methods in Aquatic Microbiology. Chichester. John Wiley. 425pp.
Bryan, Arthur, et al. 1969. Bacteriology: Principles and Practice. 6th ed. New York: Barnes and Nobles Inc. 422pp.
Cavanaugh, C.H. 1983. Symbiotic chemoautotrophic bacteria in marine invertebrates from sulfide-rich habitat. Nature 302. 56-61.
Hobbie, J.L. and J.J. Cole 1984. Response of a detrital food web to eutrophicaon. Bull. Mar Sci. 35:357-363.
Moriarty, D.J. and RSV Pullin (eds) Detritus and Microbial Ecology in Aquaculture. ICLARM Conference proceeding. Manila, Philippines
Rheinheimer, G.1991. Aquatic Microbiology. 4th ed. John Wiley and Sons. Ltd. Baffins Lane. Chichester. West Sussex. PO 19 IUD.UK.
Tortora, Funke and Case. 1992. Microbiology: An Introduction. The Benjamin Cummings Publishing Co. Inc. 810pp.